Kligler iron agar is used for the differentiation of microorganisms in the enterobacteriaceae family on the basis of dextrose and lactose. Shigella is gramnegative, nonmotile, nonspore forming, rodshaped bacteria closely related to escherichia coli and salmonella. Bailey and lacey substituted phenol red for andrade indicator previously used as ph. When these carbohydrates are fermented, the resulting production of acid is detected by the phenol red indicator. Since there is only a small amount of sugar dextrose in the medium, acid production due to its fermentation is very limited and. Food and drug administration 10903 new hampshire avenue silver spring, md 20993 1888infofda 18884636332 contact fda. Kligler iron agar is an agar medium used as a differential medium for the presumptive identification of the enterobacteriaceae based upon the fermentation of dextrose, lactose, and hydrogen sulfide production. Kligler iron agar dehydrated culture media for microbiology. Product summary and explanation kligler iron agar is a modification of kliglers original formula, recommended to identify pure cultures of colonies picked. Learn vocabulary, terms, and more with flashcards, games, and other study tools. The growth characteristics of enterobacteriaceae on tsi media are slightly different from those on kligler iron agar. Kliglers iron agar kia is used for the detection of carbohydrate fermentation.
Klebsiella pneumoniae is a gramnegative, nonmotile, encapsulated rod shaped bacterium found in the normal flora of the mouth, skin, and intestines. Many members of the familyvibrionaceae have been implicated as causative agents of diarrhea. Kliglers iron agar test was the next test that was. Oxidase testing from kliglers iron agar and triple sugar. Kligler iron agar intended use kligler iron agar is used for the identification of enterobacteria by the rapid detection of lactose and glucose fermentation with or without gas production, as well as the production of hydrogen sulfide. Glucose fermentation, h2s production, kia, kligler, lactose fermentation. What is kligler s iron agar it is a multiple test medium that will detect lactose and glucose fermentation, and the production of hydrogen sulfide resulting from the breakdown of the amino acid cysteine. If the oxidase test could be reliably performed on growth from the surface of kliglers iron agar andor triple sugar iron agar slants, it would aid in the screening of potential stool pathogens. Israel jacob kligler 24 april 1888 23 september 1944 was a microbiologist. Sugar fermentation is shown by acid production, which turns the indicator from red to yellow. Discount bbl kligler iron agar 2117 wholesale resale dealer discounts.
Kligler iron agar is a combination of the lead acetate medium described by kligler 1 and russels double sugar agar 2 and is used as a differentiation medium for typhoid, dysentery and allied bacilli 3. Kligler iron agar dehydrated culture media for microbiology physical data. This indicated a negative result and meant that the unknown organism could not. Principle, procedure and results kliglers iron agar kia is used for the detection of. The kliglers iron agar test employs a medium for the identification of enterobacteriaceae, based on double sugar fermentation and hydrogen sulphide production. Kligler iron agar kligler hajna is a solid culture medium for the identification of enterobacteriaceae, based on the fermentation of dextrose and lactose and the production of hydrogen sulfide. Kligler iron agar, modified is recommended for identification of yersinia enterocolitica. Kligler iron agar 7140 intended use kligler iron agar is used for the differentiation of microorganisms on the basis of dextrose and lactose fermentation and hydrogen sulfide production. If an organism cannot ferment glucose, then in kia, alkaline slantalkalinebutt no change reaction is observed, this reaction alone is sufficient to exclude an isolate from the enterobacteriaceae family. The methods are based on primary differentiation of the various groups of bacteria by the use of kligler iron agar and lysine iron agar. Kligler iron agar is a modification of kliglers original formula, recommended to identify pure cultures of colonies picked from primary plating media. Lab exercise 75 the kligler iron agar test65 lab exercise 76 coagulase test68 preparation for the next lab lab 8. Although both combinations enabled the presumptive recognition and differentiation of salmonella and shigella species, an analysis of data indicated that the combination of kligler iron agar and ureamotilityindole medium performed better than the combination of kligler iron agar and lysine iron agar in detecting salmonella and shigella species.
Kligler iron agar safety data sheet date of issue 11022009. Kligler iron agar is a modification of kliglers original formula, combining features of the kliglers lead acetate medium with the features of russells double sugar agar. When the test was checked, the agar had not formed a dark precipitate at the bottom of the test tube. Kligler iron agar aids in the differentiation of gramnegative enteric bacilli on the basis of their ability to ferment dextrose and lactose and produce sulfides. For identification of salmonella, shigella, and arizona group organisms from stools, triple sugar iron agar and lysineironagar are employed. Mix well and dissolve by heating with frequent agitation. Kligler iron agar kia or the identificaiton of enteric. Kligler iron agar kia test is used in differentiating certain members of the enterobacteriaceae by demonstrating hydrogen sulfide production and the fermentation of dextrose and lactose.
The medium is recommended for the identification of colonies picked off from plating media such as macconkey agar, bismuth sulphite agar, or desoxycholate. The kliglers iron agar test employs a medium for the identification of enterobacteriaceae, based on double sugar fermentation and hydrogen. Pdf biochemical characterization of bacteria responsible. Klingler iron agar combines the features of kliglers lead acetate medium 1,2,3 and russells double sugar agar 4. Evaluation of ureamotilityindole medium for recognition and. Kligler agar msds material safety data sheet or sds, coa and coq, dossiers, brochures and other available documents. A zionist and humanist, he was born in the austrohungarian empire, educated in the united states and spent most of his career in mandatory palestine, but died before the creation of the state of israel. Chemical spill kit inoculating loops, flint strikers, plastic pipettes, microscope lens cleaner and lens paper. Bailey and lacey further modified the medium by substituting phenol red indicator for. Kliglers iron agar test was the next test that was performed on the unknown isolate. Kligler iron agar kia combines features of kliglers lead acetate medium and russells double sugar agar. It can also be used for the differential identification of gramnegative enteric bacilli on the basis of the fermentation of glucose dextrose, lactose and h2s production.
Home bacteriology reactions in kligler iron agar reactions in kligler iron agar. Hardy diagnostics kligler iron agar kia is recommended for use in differentiating certain members of the enterobacteriaceae by demonstrating hydrogen sulfide production and the fermentation of dextrose and lactose. Culture and sensitivityidentification of an unknown enterobacteriaceae and antibiotic susceptibility test. The exceptions are blood agar, chocolate agar, kligler iron agar, lowensteinjensen agar, litmus milk broth, sim, and thioglycollate broth, all of which should. The production of acid by lactose andor dextrose fermentation results in color. Box 788 waterville, maine 049030788 227 china road winslow, maine 04901 technical product information kligler iron agar catalog no t1478 16 x 125 mm tube t1479 16 x 125 mm slip cap intended use.
The tsi slant is a test tube that contains agar, a phsensitive dye, 1% lactose, 1% sucrose, 0. Kligler s iron agar kia is used for the detection of carbohydrate fermentation. A differential medium for the recognition of enteric pathogens by their ability to ferment glucose andor lactose, and liberate sulphides. Bacterial isolates were initially identified to genus level by colony morphology, gram staining, catalase reaction, oxidase reaction, coagulase reaction, indole reaction, and growth on kligler iron agar, as described by cheesbrough. The methods are based on primary differentiation of the various groups of bacteria by the use of kligler iron agar and lysineironagar. In the api20e test shown below, the biogroup 1 isolate was identified as edwardsiella hoshinae, probably because biogroup 1 was not yet in the database. Kligler iron agar is used for the differentiation of microorganisms in the enterobacteriaceae family on the basis of dextrose and lactose fermentation and hydrogen sulfide production. Phenol red is the ph indicator, which exhibits a colour change in response to acid produced during the fermentation of sugars. Kligler agar used for the test comprises of casein and meat peptones that supply nitrogenous compounds and amino acids, vitamins necessary for bacterial growth. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle. Multiple drugresistant salmonella strains isolated from a.
Kligler iron agar dehydrated culture media for microbiology preparation suspend 52 grams of the medium in one litre of distilled water. Kligler iron agar k11100 safety data sheet according to 19072006ec reach, 4532010eu, 2015830eu section 4. The causative agent of human shigellosis, shigella causes. Kligler iron agar, in addition to peptone, hm peptone b and yeast extract, contains lactose and glucose dextrose, which enables the differentiation of species of enteric bacilli. Catalase test, macconkey agar test, kligler iron agar test, tween 80 hydrolysis test, kings b test was negative. Cm0033 risk phrases safety phrases ec classification this preparation is not classified as dangerous according to the ec directive 88379eec.
After the ei had been stabbed into the agar and zigzagged out of kliglers iron agar slant, it was stored for 48 hours. For identification of salmonella, shigella, and arizona group organisms from stools, triple sugar iron agar and lysine iron agar are employed. Tetracycline antibiotic revealed the highest activity with inhibition zone of 22. These strains produced acid from lactose in purple broth base and were positive in onitrophenylbetadgalactosidase onpgtest in the minitek system. Other notes components gl peptic digest of animal tissue 15. He was one of the first four professors of the hebrew university and the founder of department of hygiene and. Reactions in kligler iron agar medical laboratories. Klingler iron agar combines the features of kligler s lead acetate medium 1,2,3 and russells double sugar agar 4. Keep well closed and protected from direct sunlight and moisture. If the oxidase test could be reliably performed on growth from the surface of kligler s iron agar andor triple sugar iron agar slants, it would aid in the screening of potential stool pathogens.
Identify enterobacteriaceae on the basis of double sugar fermentation and hydrogen sulphide production using thermo scientific oxoid kligler iron agar dehydrated. Most of these organisms are nonlactose fermenters, and all are oxidasepositive. The medium is recommended for the identification of colonies picked off from plating media such as macconkey. Reactions of kia helps to includeexclude particular bacterial isolate in the family enterobacteriaceae. Handling and storage occupational exposure limits preparation europe. This can happen because utilization of sucrose in tsi agar suppresses the enzymic pathway that result in h2s production.
Oxoid kligler iron agar based on the original medium 1,2,3 combines the principles of russell 4, double sugar agar, with ferric citrate as an indicator to detect hydrogen sulphide production. It is indeed negative in tsi agar, but not in the api20e test or on kligler iron agar where there is no sucrose to be fermented to produce the excess acid to negate the h 2 s reaction. Kligler iron agar is a modification of kligler s original formula, recommended to identify pure cultures of colonies picked from primary plating media. Antimicrobialresistant bacteria in infected wounds, ghana. Kliglers iron agar test procedure, uses and interpretation.
Kligler iron agar combines the characteristics of russell double sugar agar and kligler lead acetate medium with phenol red as the dye indicator. Biochemical differentiation of the enterobacteriaceae with. History in 1911, russell described a medium containing two sugars for the isolation of typhoid bacilli in urine. The medium is recommended for the identification of colonies picked off from plating media such as macconkey agar, bismuth sulphite agar, or desoxycholate citrate agar, etc. However, when lactose is fermented, the large amount of acid produced, avoids reoxidation and therefore the entire medium turns yellow. In 1918, kligler described a medium for detection of h 2 s and differentiation of salmonella spp. Hajna developed the formulation for tsi agar by adding sucrose to the double sugar glucose and lactose formulation of kligler iron agar.
The tsi slant is a test tube that contains agar, a phsensitive dye phenol red, 1% lactose, 1% sucrose, 0. Reactions of kia helps to includeexclude particular bacterial. Kligler iron agar kia or the identificaiton of enteric bacteria. In 1911, russell 1 described a combination of two sugars in an agar medium to differentiate gram negative intestinal microorganisms. Make a suspension of 52 g in 1 l of distilled water. Kliglers iron agar test was the next test that was performed.
Acute and delayed effects are indicated in sections 2 and 11. Dehydrated culture media of pinkish beige colour preparation. The original medium was a soft nutrient agar containing dextrose, andrade indicator and lead acetate. Dispense into tubes and sterilize in autoclave at 121. The usefulness of this schema for diagnostic bacteriology. The basal medium of kia is composed of casein and meat peptones with the addition of lactose and dextrose. It will cause the agar to breakdown and become unusable. As mentioned in theory, the fermentation readings with tsia and kia must take place between 18 and 24 hours after inoculation. What is kliglers iron agar it is a multiple test medium that will detect lactose and glucose fermentation, and the production of hydrogen sulfide resulting from the breakdown of the amino acid cysteine. Evaluation of ureamotilityindole medium for recognition.
Showing biochemical test of isolated bacteria, a sim test, b simmons citrate test, c kligler iron agar test, d triple sugar iron test, e urease test. Strain 11925 showed an acid butt and alkaline slant on kiagar, with a small quantity of hydrogen sulfide. Heat stirring constantly to boiling point and boil for 1 minute. Most tube media both broth and agar can be stored at room temperature for about two months. Distribute into test tubes and sterilize at 121c for 15 minutes.
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